Jeffrey R. Morgan, Martin L. Yarmush - Tissue Engineering

(Methods in Molecular Medicine) Jeffrey R. Morgan, Martin L. Yarmush - Tissue Engineering -Humana Press (1998)

Certain analogs of the extracellular matrix (ECM) have been shown to possess surprising morphogenetic activity during healing of lessons m various anatomical sites. This chapter describes methods for synthesis of the two ECM analogs that have been studied most extensively. The reader is referred to descriptions of these methods m the original literature (1-3) The biological activity of ECM analogs has been reviewed elsewhere (4). One of these analogs, referred to as the skin regeneration template (SRT), has Induced regeneration of dermis in full-thickness skin wounds m the guinea pig model (2,.5-7), the porcine model (8), and in humans (5,9-11).

Since it is well known that the dermis of the adult mammal does not regenerate spontaneously (12,13), the SRT is required for dermal regeneration in all commonly encountered skin wounds that are sufficiently deep to have compromised the dermis. The SRT is currently used as a dermal regeneration treatment for patients who have sustained deep burns or deep mechanical trauma, including trauma from elective surgery, and who would otherwise have been treated with auto-grafts (10)

In the clinical setting or in animal models, the SRT is applied on wounds as a bilayer graft; the proximal layer is the highly porous ECM analog and the distal layer is a silicon film (Fig. 1). The latter has no biological activity, but serves as a temporary dressing that protects the proximal layer from dehydration and bacterial invasion, and also converts the bi-layer into a mechanically competent sheet, capable of being handled conveniently and sutured on the patient’s tissues

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